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KMID : 0613820040140010174
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Journal of Life Science
2004 Volume.14 No. 1 p.174 ~ p.179
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Isolation and Characteristic of the Phosphate Solubilizing Bacteria Klebsiella sp. DA 71-1
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Lee Jin-Woo
Jung Youn-Ju
Lee Kyung-Ah
Choi Si-Lim
Kim Young-Gil
Choi Yong-Lark
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Abstract
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To develop high effiency biofertilizer, a bacterium having ability to solubilize inorganic phosphate was isolated from cultivated soils, using a sucrose minimal agar-hydroxyapatite medium. The strain was identified as Klebsiella sp. DA71-1, based on the physiological and biochemical properties. The activity of solubilizing inorganic phosphate of Klebsiella sp. DA71-1 against three types of insoluble phosphate such as tri-calcium phosphate, aluminium phosphate, hydroxyapatite were quantitatively determined. The results indicated that the strain solubilized hydroxyapatite. The MPS (mineral phosphate solubilizing) conditions of Klebsiella sp. DA71-1, were measured to determine the optimal conditions. The optimal temperature and initial pH to solubilize insoluble phosphate in sucrose minimal medium were 30℃ and pH 6.0, respectively.
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KEYWORD
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MPS, hydroxyapatite, insoluble phosphate, Klebsiella sp
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